Technical Data
A3774-10B
ASC2 (Activating Signal Cointegrator-2, NCOA6, NCOA-6, Nuclear Receptor Coactivator 6, PRIP, Peroximome-Proliferator-Activated Receptor Interacting Protein, AIB3, Pyrin Domain Containing 1, POP1)
Description:
Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Coactivates expression in an agonist- and AF2-dependent manner. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ERs), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Probably functions as a general coactivator, rather than just a nuclear receptor coactivator. May also be involved in the coactivation of the NF-kappa-B pathway. May coactivate expression via a remodeling of chromatin and its interaction with histone acetyltransferase proteins. Involved in placental, cardiac, hepatic and embryonic development. NCOA6 is over-expressed in several breast cancer cell lines.

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
A synthetic peptide that maps to a region between residues 1050 and 1100 of human Activating Signal Cointegrator-2 using the numbering given in Swiss-Prot entry Q14686 (GeneID 23054).
Purity:
Serum
Form
As reported
Specificity:
Specific for human ASC2 protein. Other species not tested. Reactivity with mouse ASC2 is expected owing to conservation of sequence between this ortholog and human ASC2 at the site to which the epitope maps.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.