		Technical Data
A3785-20	Asialo GM1 (Asialoganglioside GM1)

Description:
A member of the family of glycosphingolipids, monosialoanglioside acts as a receptor and antigen for cholera. GM1 ganglioside, one of the glycosphingolipids widely distributed in all tissues, occurs in highest concentrations in the central nervous system. It is primarily located in the outer surface of the mammalian cell's plasma membrane and in synaptic membranes of the CNS. GM1 ganglioside modulates a number of cell surface and receptor activities as well as neuronal differentiation and development, protein phosphorylation and synaptic function.

Applications:
Suitable for use in ELISA and TLC Immunoblot. Other applications have not been tested.

Recommended Dilutions:
ELISA: 1:1600 (~50ng antigen optimal detection)
TLC: 1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice		Rabbit

Concentration:

Not Determined

Immunogen:

Asialo GM1

Purity:

Serum

Form

Supplied as a liquid. No preservative added.

Specificity:

Recognizes Asialo GM-1. No crossreactivity with other carbohydrate epitopes.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Yoshino, H., et al., J. Neurochemistry, 61: 658 (1993). 2. Kusunoki, S., et al., Neurology, 37: 1,795 (1987).