Technical Data
A3825-12
Asparagine Synthetase (AS, Cell Cycle Control Protein TS11, Glutamine Dependent Asparagine Synthetase 3, Glutamine Hydrolyzing, TS11)
Description:
Asparagine synthetase (AS) is a housekeeping enzyme responsible for the production of asparagine from aspartate and glutamine. Most mammalian cells express AS and regulate the level of activity in response to the concentration of asparagine in the medium. Certain tumor cells, in contrast, exhibit little or no AS activity and rely on the surrounding medium as a source of exogenous asparagines. Therefore tumor cells may be selectively killed by a chemotherapeutic drug using asparaginase. This approach has been exploited in the treatment of certain cancers like childhood acute lymphoblastic leukemia.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and IImmunofluorescence/Immunocytochemistry. Not recommended for use in FACS or Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000-1:2000
Immunohistochemistry (Paraffin): 1:50
Immunofluorescence (IC): 1:50-1:100
Optimal dilutions to be determined by the researcher.

Positive Controls:
K562 cell lysate, human breast carcinoma tissue and human muscle tissue

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Manufactured incorporating RabMAb® technology under Epitomics US patent, No 5,675,063, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG6k30Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to residues on the C-terminus of human Asparagine Synthetase.
Purity:
Supernatant
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.01% sodium azide, 50% glycerol.
Specificity:
Recognizes human Asparagine Synthetase at ~60kD. Species Crossreactivity: rat and mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Gantt, J.S., et al., J. Biol. Chem. 255: 4808-4813 (1980). 2. Ortega, J.A., et al., Cancer Res. 37: 535-540 (1977).