Technical Data
Aspartate Aminotransferase 1, GOT1 (Growth-inhibiting protein 18, GIG18, Glutamic-oxaloacetic transaminase 1)
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in aa metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:16000
Western Blot: 0.5-1.5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabAffinity Purified
100ug-20CBlue IceHumanGoat
Synthetic peptide corresponding to C-TADFREDPDPRKVN, aa22-35 of human Aspartate Aminotransferase 1.
Purified by immunoaffinity chromatography.
Supplied as a liquid in tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Recognizes human Aspartate Aminotransferase 1. Species sequence homology: Mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Teichberg VI.From the liver to the brain across the blood-brain barrier. Proc Natl Acad Sci U S A. 2007 May 1;104(18):7315-6. Epub 2007 Apr 26. No