Technical Data
A3879-06D1
Aspartate Aminotransferase 1, GOT1 (Growth-inhibiting protein 18, GIG18, Glutamic-oxaloacetic transaminase 1)
Description:
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in aa metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:16000
Western Blot: 0.5-1.5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-TADFREDPDPRKVN, aa22-35 of human Aspartate Aminotransferase 1.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:
Recognizes human Aspartate Aminotransferase 1. Species sequence homology: Mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Teichberg VI.From the liver to the brain across the blood-brain barrier. Proc Natl Acad Sci U S A. 2007 May 1;104(18):7315-6. Epub 2007 Apr 26. No