Technical Data
A3879-25
Aspartate Aminotransferase 2 (ASPATA, AL022787, Fatty Acid-binding Protein, FABP 1, FABP-1, FABPpm, Glutamate Oxaloacetate Transaminase 2, Glutamic Oxaloacetic Transaminase 2 Mitochondrial (Aspartate Aminotransferase 2), GOT 2, GOT2, GOT-2, Kynurenine Ami
Description:
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq]

Epitope: CKDADEAKRVES

Theoretical Molecular Weight:
47.5kDa

Applications:
Suitable for use in ELISA and Westrern Blot. Other applications not tested.

Recommended Dilution:
ELISA: Antibody detection limit dilution 1:32,000.
Western Blot: 0.03-0.1ug/ml, Approx 45kD band observed in Human Kidney and Liver lysates (calculated MW of 47.5kDa according to NP_002071.2).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 3 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Peptide with sequence CKDADEAKRVES, from the internal region of the protein sequence according to NP_002071.2
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris-saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human Aspartate Aminotransferase 2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Teichberg VI. From the liver to the brain across the blood-brain barrier. Proc Natl Acad Sci U S A. 2007 May 1;104(18):7315-6. PMID: 17463093