Technical Data
A3886
ADMA (Asymmetric Dimethylarginine)
Description:
Asymmetric dimethylarginine (ADMA) is a naturally occurring chemical found in blood plasma. It is a metabolic by-product of continual protein modification processes in the cytoplasm of all human cells. It is closely related to L-arginine, a conditionally-essential amino acid. ADMA interferes with L-arginine in the production of nitric oxide, a key chemical involved in normal endothelial function and, by extension, cardiovascular health.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Crossreactivity:
Using conjugated ADMA-KLH, antibody specificity was performed with an ELISA test by competition experiments with the following compounds:
Compound: Cross-reactivity ratio(a)
ADMA-BSA: 1
SDMA-BSA: 1:>100
NDMA-BSA: 1:>100
L-Arginine-BSA: 1:>5000

(a) ADMA-BSA concentration/unconjugated or conjugated amino acids concentration at half displacement
BSA=Bovine Serum Albumin

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgG1Affinity Purified
SizeStorageShippingSourceHost
10ug-20CBlue IceRabbit
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
ADMA, conjugated to KLH.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from 50mM Tris, pH 7.4. Reconstitute with sterile ddH2O to a concentration of 1mg/ml.
Specificity:
Recognizes ADMA.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.