Technical Data
ATG4B (Cysteine Protease ATG4B, AUT-like 1 Cysteine Endopeptidase, Autophagin-1, Autophagy-related Cysteine Endopeptidase 1, Autophagy-related Protein 4 Homolog B, hAPG4B, APG4B, AUTL1, KIAA0943)
AG4B is a 393aa cysteine protease consisting of the active catalytic triad site of Cys74, His280 and Asp278 and belonging to the peptidase C54 family. It is required for autophagy, which is essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death and aging. It negatively regulates the localization of LC3 to a membrane compartment by delipidation and mediates murine Atg8L/Apg8L modification during the formation of autophagosomes. APG4B is expressed in skeletal muscle, brain, heart, liver and pancreas.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-3ug/ml
Immunohistochemistry (paraffin): 10ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa and RAW cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
Synthetic peptide corresponding to aa350-400 of human APG4B.
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Recognizes human APG4B. Species Crossreactivity: rat, mouse. Species sequence homology: pufferfish, zebrafish, xenopus, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kumanomidou, T. et al. J. Mol. Biol. 355:612-618 (2006). 2. Sou, YS. et al. FEBS J. 273:2553-2562 (2006). 3. Tanida, I. et al. J. Biol. Chem. 279:36268-36276 (2004).