		Technical Data
A3935-35	ATG7 (APG7-Like, APG7L, ATG7 Autophagy-related 7 Homolog (S. cerevisiae), Autophagy Related Protein 7, DKFZp434N0735, GSA7, hAGP7, Ubiquitin Activating Enzyme E1-like Protein)

Description:
Applications:
Suitable for use in ELISA, Immunohistochemistry (paraffin), and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin): 10ug/ml
ELISA: 1:15,000-1:75,000
Western Blot: 1:500-1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		4°C/-20°C (No Glycerol)	Blue Ice	Human	Rabbit

Concentration:

~1mg/ml

Immunogen:

Synthetic peptide sequence corresponding to aa sequence 558-569 of human ATG7 protein.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.

Specificity:

Recognizes human ATG7.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.