Technical Data
A3935-35A
ATG7 (APG7L, Ubiquitin-like Modifier-activating Enzyme ATG7, ATG12-activating Enzyme E1 ATG7, Autophagy-related Protein 7, APG7-like, hAGP7, Ubiquitin-activating Enzyme E1-like Protein)
Description:
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway, is important for normal growth control and may be defective in tumor cells. A member of the autophagy family of proteins is APG7L which was identified in yeast as a ubiquitin-E1-like enzyme; this function is conserved in the mammalian homolog. In mammalian cells, APG7L is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles. The protein thus plays an indispensable role in the initial step of the conjugation system. APG7L interacts with Apg8p/Aut7p and Aut1p:Apg3p in addition to Apg12p. The protein maps to 3p25.3-p25.2 region of human chromosome. Ablation of the protein leads to abnormal swellings and dystrophy of purkinje cell axon terminals in the deep cerebellar nuclei (DCN).

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined.
Immunogen:
Synthetic peptide corresponding to aa28-51 (HELTQKKLNEYRLDEAPKDIKGYY) of human APG7L.
Purity:
Serum
Form
Supplied as a liquid, 0.025% sodium azide, 50% glycerol.
Specificity:
Recognizes human APG7L. Species Crossreactivity: monkey and rat. Species sequence homology: chicken.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Masaaki, K. et al. PNAS. 104:14489-14494 (2007). 2. Isei, T. et al. J. Biol. Chem. 276:1701-1706 (2001). 3. Gozuacik, D. et al. Oncogene. 23:2891-2906 (2004). 4. Kisen, GO. et al. Carcinogenesis. 14:2501-2505 (1993). 5. Komatsu, M. et al. J. Cell. Biol. 169:425-434 (2005).