		Technical Data
A3935-35A	ATG7 (APG7L, Ubiquitin-like Modifier-activating Enzyme ATG7, ATG12-activating Enzyme E1 ATG7, Autophagy-related Protein 7, APG7-like, hAGP7, Ubiquitin-activating Enzyme E1-like Protein)

Description:
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway, is important for normal growth control and may be defective in tumor cells. A member of the autophagy family of proteins is APG7L which was identified in yeast as a ubiquitin-E1-like enzyme; this function is conserved in the mammalian homolog. In mammalian cells, APG7L is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles. The protein thus plays an indispensable role in the initial step of the conjugation system. APG7L interacts with Apg8p/Aut7p and Aut1p:Apg3p in addition to Apg12p. The protein maps to 3p25.3-p25.2 region of human chromosome. Ablation of the protein leads to abnormal swellings and dystrophy of purkinje cell axon terminals in the deep cerebellar nuclei (DCN).

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

Not determined.

Immunogen:

Synthetic peptide corresponding to aa28-51 (HELTQKKLNEYRLDEAPKDIKGYY) of human APG7L.

Purity:

Serum

Form

Supplied as a liquid, 0.025% sodium azide, 50% glycerol.

Specificity:

Recognizes human APG7L. Species Crossreactivity: monkey and rat. Species sequence homology: chicken.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Masaaki, K. et al. PNAS. 104:14489-14494 (2007). 2. Isei, T. et al. J. Biol. Chem. 276:1701-1706 (2001). 3. Gozuacik, D. et al. Oncogene. 23:2891-2906 (2004). 4. Kisen, GO. et al. Carcinogenesis. 14:2501-2505 (1993). 5. Komatsu, M. et al. J. Cell. Biol. 169:425-434 (2005).