Technical Data
A3935-70
ATG14 (Autophagy-related Protein 14-like Protein, Atg14L, Beclin 1-associated Autophagy-related Key Regulator, BARKOR, KIAA0831, MGC126291, MGC126292)
Description:
Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but is also associated with a number of physiological processes including development, differentiation, neurodegeneration, infection and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and is directed by a number of autophagy-related (Atg) genes. These proteins are involved in the formation of autophagosomes, cytoplasmic vacuoles that are delivered to lysosomes for degradation. The class III type phosphoinositide 3-kinase (PI3K) Vps34 regulates vacuolar trafficking and autophagy (4,5). Multiple proteins
associate with Vsp34, including p105/Vsp15, Beclin-1, UVRAG, Atg14, and Rubicon, to determine Vsp34 function (6-12). Atg14 and Rubicon were identified based on their ability to bind to Beclin-1 and participate in unique complexes with opposing functions (9-12). Rubicon, which localizes to the endosome and lysosome, inhibits Vps34 lipid kinase activity; knockdown of Rubicon enhances autophagy and endocytic trafficking (11,12). In contrast, Atg14 localizes to autophagosomes, isolation membranes and ER, and can enhance Vps34 activity. Knockdown of Atg4 inhibits starvation-induced autophagy (11,12).

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4C with gentle shaking, overnight.
Immunoprecipitation: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to a region surrounding Val215 of human Atg14.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
Specificity:
endogenous levels of total Atg14. Species Crossreactivity: monkey, mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1) Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21. (2) Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18. (3) Levine, B. and Yuan, J. (2005) J Clin Invest 115, 2679-88.
(4) Corvera, S. (2001) Traffic 2, 859-66 (5) Yan, Y. and Backer, J.M. (2007) Biochem Soc Trans 35,
239-41. (6) Stack, J.H. et al. (1995) J Cell Biol 129, 321-34. (7) Zeng, X. et al. (2006) J Cell Sci 119, 259-70. (8) Liang, C. et al. (2006) Nat Cell Biol 8, 688-99. (9) Itakura, E. et al. (2008) Mol Biol Cell 19, 5360-72. (10) Sun, Q. et al. (2008) Proc Natl Acad Sci U S A 105, 19211-6. (11) Zhong, Y. et al. (2009) Nat Cell Biol 11, 468-76. (12) Matsunaga, K. et al. (2009) Nat Cell Biol 11, 385-96.