Technical Data
ATH 1 (MATH 1, NeuroD Family, Helix-loop-helix Transcription Factor)
ATH 1 (MATH 1, NeuroD Family) belongs to the basic helix-loop-helix (BHLH) family of transcription factors. It activates E-box dependent transcription.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot (ECL): 1:200-1:1000
Immunohistochemistry: 1:200-1:1000
Optimal dilutions to be determined by the researcher.

Recommended Control Peptide: A3950-01A

1. Dissect tissues and freeze on dry ice.
2. Cut on a cryostat -10um slices.
3. Dry slides at RT for 30-90 min.
4. Fix with cold Acetone/Methanol (50/50) for 2 min.
Either process further or store at -20C. Alternative is fixation with 4% PFA and treatment with a trypsin solution. (0.05% trypsin, incubation for 10 minutes at 37C, followed by 3 washes with PBS).
5. Let air dry.
6. Treat with PBS for 5 min.
7. Incubate in 50mM ammonium chloride for 30 min.
8. Treat with PBS for 5 min.
9. Treat with Blocking serum for 30-45 min.
10. Treat with A3950-01 for 90 min.
11. Wash 3 times for 5 min.
12. Secondary fluorescent conjugate antibody for 30 min.
13. Wash 3 times for 5 min and mount.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
Synthetic peptide corresponding to aa188-200, SFNNDKKLSKYET of human ATH1.
Purified by affinity chromatography.
Supplied as a liquid in PBS, pH 7.2. No preservative added.
Recognizes human ATH 1 at ~38kD. Species Crossreactivity: rodent.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.