Technical Data
A3999-23
ATPase/GTPase Assay Kit, BioAssay™
1Kit
Kits and Assays Storage: 4°CShipping: Blue Ice
ATPases and GTPases catalyze the decomposition of ATP or GTP into ADP or GDP and free phosphate ion. These enzymes play key roles in transport, signal transduction, protein biosynthesis and cell differentiation.
ATPase/GTPase Assay Kit offers a highly sensitive method for determining ATPase/GTPase activities in a microplate format. Its proprietary formulation features a single reagent for accurate determination of enzyme activity in 30 minutes at RT. The improved malachite green reagent forms a stable dark green color with liberated phosphate, which is measured on a plate reader (600-660nm).

Key Features:
High Sensitivity: Detection of 0.007U/L ATPase or GTPase activity.
Fast and Convenient: Single reagent, homogeneous “mix-and-measure” assay allows quantitation of enzyme activity within 30 minutes.
Robust and Amenable to HTS: Detection at 620nm greatly reduces potential interference by colored compounds. Z’ factors of >0.7 are observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems.

Applications:
Determination of ATPase and GTPase activity.
Drug Discovery: High-throughput screen for ATPase or GTPase inhibitors.

Kit Components:
A3999-23A: Reagent, 1x50ml
A3999-23B: Assay Buffer:,1x10ml
A3999-23C: Standard (1mM phosphate), 1x1ml

Storage and Stability:
Store components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Precautions: A3999-23A: Reagent contains 0.27M H2SO4. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.