Technical Data
A3999-67
ATP5B, ID (ATP Synthase Subunit beta, Mitochondrial, ATPSB, ATPMB)
Description:
ATP5B is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). It is the beta subunit of the catalytic core.

Applications:
Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:1000
Western Blot: 1:100-1:500
Flow Cytometry: 1:10-1:50
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to a portion of amino acids within aa142-171 from the center region of human ATP5B conjugated to KLH.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human ATP5B.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Neckelmann,N., et.al., Genomics 5(4), 829-843 (1989) 2. Ohta,S., et.al., J. Biol. Chem. 263(23), 11257-11262 (1988) 3. Wallace,D.C., et.al., Curr. Genet. 12(2), 81-90 (1987).