		Technical Data
A3999-86J	ATP1B1 (Sodium/Potassium-transporting ATPase Subunit beta-1, Sodium/Potassium-dependent ATPase Subunit beta-1, ATP1B)

Description:
This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:128,000
Western Blot: 0.1-0.3ug/ml, rat retina lysates and 50kDa in mouse and rat kidney lysates observed on ~48kD bands
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to C-KTEISFRPNDPKSYE, from ATP1B1, at the internal region of the protein (NP_001668.1, NP_001001787.1).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Specificity:

Recognizes ATP1B1 Species Crossreactivity: Mouse and rat. Species sequence homology: Human, bovine, canine and porcine.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Ushkaryov YuA, Monastyrskaya GS, Broude NE, Nikiforova NN, Bessarab DA, Orlova MYu, Petrukhin KE, Modyanov NN, Sverdlov ED Human Na+,K+-ATPase genes. Beta-subunit gene family contains at least one gene and one pseudogene FEBS Lett. 1989 Nov 257(2):439-42.