Technical Data
A3999-87
ATP12A, ID (Potassium-transporting ATPase alpha Chain 2, Proton Pump, Non-gastric H(+)/K(+) ATPase Subunit alpha, ATP1AL1)
Description:
ATP12A belongs to the family of P-type cation transport ATPases. This protein is a catalytic subunit of the ouabain-sensitive H+/K+ -ATPase that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for potassium absorption in various tissues.

Applications:
Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 1:1000
Flow Cytometry: 1:10:1:50
Immunohistochemistry (paraffin): 1:50-1:100
Western Blot: 1:100-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa839-868 in the center region of human ATP12A.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human ATP12A.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Li,J., et.al., Kidney Int. 65(4), 1301-1310 (2004).