Technical Data
A4000-72B
ATPase beta 1, Na, K
Description:
The Na,K-ATPase is an integral membrane protein complex responsible for establishing the electrochemical gradients of Na+ and K+ ions across the plasma membranes of mammalian cells. The enzyme consists of an a and b subunit, both of which exist as isoforms. The a subunit is believed to be the catalytically active subunit, while the b subunit appears necessary for the assembly and transport of the sodium pump to the plasma membrane. The beta subunit is a membrane spanning glycoprotein present in all cell types. The antigenic determinant of this antibody has been mapped to the ectodomain (~residues 62-304) of the beta subunit.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:250
Immunohistochemistry: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a6D136Supernatant
SizeStorageShippingSourceHost
200ul-20CBlue IceSheepMouse
Concentration:
Not Determined
Immunogen:
Purified sheep kidney Na+/K+ ATPase-beta.
Purity:
Supernatant
Form
Supplied as a liquid PBS, 0.05% sodium azide.
Specificity:
Recognizes the beta1 subunit of sheep Na+/K+ ATPase-beta. Species Crossreactivity: human, mouse, porcine and canine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.