		Technical Data
A4000-72B	ATPase beta 1, Na, K

Description:
The Na,K-ATPase is an integral membrane protein complex responsible for establishing the electrochemical gradients of Na+ and K+ ions across the plasma membranes of mammalian cells. The enzyme consists of an a and b subunit, both of which exist as isoforms. The a subunit is believed to be the catalytically active subunit, while the b subunit appears necessary for the assembly and transport of the sodium pump to the plasma membrane. The beta subunit is a membrane spanning glycoprotein present in all cell types. The antigenic determinant of this antibody has been mapped to the ectodomain (~residues 62-304) of the beta subunit.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:250
Immunohistochemistry: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2a	6D136	Supernatant

Size		Storage	Shipping	Source	Host
200ul		-20°C	Blue Ice	Sheep	Mouse

Concentration:

Not Determined

Immunogen:

Purified sheep kidney Na+/K+ ATPase-beta.

Purity:

Supernatant

Form

Supplied as a liquid PBS, 0.05% sodium azide.

Specificity:

Recognizes the beta1 subunit of sheep Na+/K+ ATPase-beta. Species Crossreactivity: human, mouse, porcine and canine

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.