		Technical Data
A4000-80	ATPase H+ Transporting Lysosomal Accessory Protein 1 (ATP6AP1, 16A, Ac45, ATPase H+ Transporting Lysosomal (Vacuolar Proton Pump) Subunit 1, ATP6S1, ATPase H+ Transporting Lysosomal Interacting Protein 1, ATP6IP1, CF2, H-ATPase Subunit, MGC129781, Protein

Description:
This gene encodes a component of a multisubunit enzyme (1mD MW) that mediates acidification of eukaryotic intracellular organelles. Vacuolar ATPase (V-ATPase) is comprised of a cytosolic V1 (site of the ATP catalytic site) and a transmembrane V0 domain. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. The encoded protein of this gene is approximately 45kD and may assist in the V-ATPase-mediated acidification of neuroendocrine secretory granules. [provided by RefSeq]

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
ELISA: 3ng/ml-1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1,k	10B237	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~0.5mg/ml

Immunogen:

Partial recombinant protein corresponding to a portion of aa51-151 of human ATP6AP1 (NP_001174), with GST tag. MW of GST tag alone is 26kD.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free).

Specificity:

Recognizes human ATP6AP1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.