Technical Data
A4000-94
ATPase 2, Ca2+ Transporting, Sarcoplasmic Endoplasmic Reticulum (SERCA2)
Description:
ATP dependent calcium pumps are responsible, in part, for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium (SERCA) ATPases. The SERCA1 gene is exclusively expressed in type II (fast) skeletal muscle. The SERCA2 gene is subject to tissue dependent processing which is responsible for the generation of SERCA2a muscle-specific form expressed in type I (slow) skeletal, cardiac and smooth muscle and the SERCA2b isoform expressed in all cell types. The SERCA3 gene is not as well characterized and is found in non-muscle cells.

Applications:
Suitable for use in ELISA, Immunoprecipitation, Blocking/Neutralization, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:2500 Detects an ~110kD protein representing SERCA2 ATPase from canine skeletal muscle triad preparations. Not suitble for Western Blot detection of rat SERCA2.
Immunohistochemistry (Frozen): 1:500
Immunohistochemistry (paraffin): 1:10-1:2000
Immunofluorescence (IC): 1:10-1:2000 Staining of SECRA2 ATPase in rabbit skeletal muscle results in strong labeling of the entire type I
(slow) myofiber consistent with sarcoplasmic reticulum localization.
Blocking/neutralization: 50-200 molar excess
ELISA: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG14i351Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceCanineMouse
Concentration:
~1mg/ml
Immunogen:
Purified canine cardiac sarcoplasmic reticulum
Purity:
Puriifed by affinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity:
Recognizes canine Sarcoplasmic or Endoplasmic Reticulum Calcium 2 (SERCA2) ATPase. Does not discriminate between the two isoforms. Species Crossreactivity: human, rabbit, rat, bovine, porcine, mouse, amphibian, canine, guinea pig, hamster, primate, sheep and virus
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Leatherbury L, Yu, Q, et al., Am J Physiol Heart Circ Physiol April 18, 2008:00160.2007