Technical Data
A4001-35A
ATP-Citrate Lyase, CT (ACLY, ATP Citrate (pro-S) Lyase, ATP Citrate Synthase, Citrate Cleavage Enzyme, EC 2.3.3.8)
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Technical Data |
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A4001-35A |
ATP-Citrate Lyase, CT (ACLY, ATP Citrate (pro-S) Lyase, ATP Citrate Synthase, Citrate Cleavage Enzyme, EC 2.3.3.8) |
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Description: ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:1,000 Western Blot: 1:50-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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