		Technical Data
A4150	Atrial Natriuretic Peptide (ANP, Atrial Natriuretic Factor)

Description:
Atrial natriuretic peptide (ANP), atrial natriuretic factor (ANF), or atriopeptin, is a polypeptide hormone secreted by atrial myocytes that is involved in the homeostatic control of body water, sodium, potassium and adiposity. It is released by atrial myocytes, muscle cells in the atria of the heart, in response to high blood pressure. ANP acts to reduce the water, sodium and adipose loads on the circulatory system, thereby reducing blood pressure.

Applications:
Suitable for use in ELISA, Immunohistochemistry, RIA, liquid phase assay without plasma extraction. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: Formalin-fixed paraffin embedded tissue
RIA: Competetive radioimmunoassay for the clinical measurement of circulating ANP levels in conjunction with cyclic GMP.
Optimal dilutions to be determined by the researcher.

Hybridoma:
NSO myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	8.B.4	Affinity Purified

Size		Storage	Shipping	Source	Host
1mg		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Atrial Natriuretic Peptide whole molecule (BSA).

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, 10mM sodium azide.

Specificity:

Recognizes whole molecule and aa4-28 of human Atrial Natriuretic Peptide.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Arbustini, E., et al., Am. J. Cardiol. 66: 973-980 (1990).