Technical Data
A4150-01
Atrial Natriuretic Peptide (HRP) (ANP, Atrial Natriuretic Factor)
Description:
Applications:
Suitable for use in ELISA and Immunohistochemistry. Also suitable for use in liquid phase assay without plasma extraction. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: Formalin-fixed paraffin embedded tissue
Optimal dilutions to be determined by the researcher.

Hybridoma:
Hybridization of NSO myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
TypeIsotypeCloneGrade
MabIgG18.B.4Affinity Purified
SizeStorageShippingSourceHost
100ug4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
As Reported
Immunogen:
ANP (whole molecule and residues 4-28).
Purity:
Purified by Protein A chromatography.
Form
Supplied as a liquid in PBS. No stabilizing proteins and no preservative added. Conjugated to horseradish peroxidase (HRP).
Specificity:
Recognizes whole molecule and aa4-28 of Human Atrial Natriuretic Peptide.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Arbustini, E., et al., Am. J. Cardiol. 66: 973-80 (1990).