Technical Data
A4152-35
Atrial Natriuretic Peptide (ANP, Atrial Natriuretic Factor)
Description:
Atrial natriuretic peptide (ANP), atrial natriuretic factor (ANF) or atriopeptin, is a polypeptide hormone secreted by atrial myocytes that is involved in the homeostatic control of body water, sodium, potassium and adiposity. It is released by atrial myocytes, muscle cells in the atria of the heart, in response to high blood pressure. ANP acts to reduce the water, sodium and adipose loads on the circulatory system, thereby reducing blood pressure.

Applications:
Suitable for use in Immunohistochemistry, Immunoprecipitation, RIA, ELISA and Immunofluorescence. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: 2-10ug/ml
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies (IF):
I1904-42Q: IgG, H&L (FITC) Pab Dk x Rb
I1904-46H: IgG, H&L, X-Adsorbed (FITC) Pab Dk x Rb
I1904-46C: IgG, H&L (FITC) Pab Dk x Rb

Recommended Secondary Antibodies (Avidin):
I1904-40H: IgG, Fc (Biotin) Pab Gt x Rb
I1904-41C: IgG, F(ab')2 (Biotin) Pab Gt x Rb

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceRatRabbit
Concentration:
~1mg/ml
Immunogen:
Rat ANP conjugated to BSA.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in Ammonia Acetate, 0.6% BSA, 0.1% sodium azide.
Specificity:
Recognizes rat Atrial Natriuretic Peptide (ANP). Species Crossreactivity: human and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kehat, I., et al., The Journal of Clinical Investigation 108: 407-414 (2001). 2. Cho, E.E., et al., Arch. Oral Biol. 45: 425-430 (2000). 3. Ohmori, Y., Horm. Metab. Res. 30: 384-388 (1998). 4. Maley, B.E., Chem. Senses 21: 367-376 (1996). 5. Jougasaki, M., et al., Am. J. Physiol. 268: F657-663 (1995). 6. McKenzie, J.C., J. Histochem Cytochem. 40: 1211-1222 (1992). 7. Kawaguchi, S., et al., J. Histochem. Cytochem. 37: 1739-1742 (1989). 8. Yamada, H., et al., Histochemistry 89: 411-413 (1988). 9. Chapeau, C., et al., J. Histochem. Cytochem. 33: 541-550 (1985). 10. Cantin, M., et al., Histochemistry 80: 113-127 (1984).