		Technical Data
A4153-05	Atrial Natriuretic Peptide, alpha (ANP, Atrial Natriuretic Factor, ANF, ATFB6, CDD-ANF, Natriuretic Peptide A, NPPA, Prepronatriodilatin, PND)

Description:
Applications:
Suitable for use in Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:200
Immunohistochemistry (formalin-fixed paraffin): 1:500 (ABC, DAB)
Optimal dilutions to be determined by the researcher.

Positive Control:
Rat or Mouse heart tissue

AA Sequence:
Ser-Leu-Arg-Arg-Ser-Ser-Cys-Phe-Gly-Gly-Arg-Met-Asp-Arg-Ile-Gly-Ala-Gln-Ser-Gly-Leu-Gly-Cys-Asn-Ser-Phe-Arg-Tyr

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
50ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

Not determined

Immunogen:

Synthetic peptide corresponding to aa1-28 of human alpha-ANP.

Purity:

Serum

Form

Supplied as a lyophilized powder. Reconstitute with sterile dH2O.

Specificity:

Recognizes human alpha-ANP. Species crossreactivity: a-ANP (aa1-28) (human, sheep, canine, porcine), human b-ANP, Urodilatin. Sequence homology (<1%): Rat ANP, human BNP-32, Endothelin-1 (human, rat, porcine, mouse, canine, bovine), CNP (aa24-53), porcine BNP-26, rat BNP-45.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.