Technical Data
A4153-05
Atrial Natriuretic Peptide, alpha (ANP, Atrial Natriuretic Factor, ANF, ATFB6, CDD-ANF, Natriuretic Peptide A, NPPA, Prepronatriodilatin, PND)
Description:
Applications:
Suitable for use in Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:200
Immunohistochemistry (formalin-fixed paraffin): 1:500 (ABC, DAB)
Optimal dilutions to be determined by the researcher.

Positive Control:
Rat or Mouse heart tissue

AA Sequence:
Ser-Leu-Arg-Arg-Ser-Ser-Cys-Phe-Gly-Gly-Arg-Met-Asp-Arg-Ile-Gly-Ala-Gln-Ser-Gly-Leu-Gly-Cys-Asn-Ser-Phe-Arg-Tyr

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to aa1-28 of human alpha-ANP.
Purity:
Serum
Form
Supplied as a lyophilized powder. Reconstitute with sterile dH2O.
Specificity:
Recognizes human alpha-ANP. Species crossreactivity: a-ANP (aa1-28) (human, sheep, canine, porcine), human b-ANP, Urodilatin. % Cross-reactivity with Peptides: a-ANP (1-28) (Human, Ovine, Canine): 100%; b-ANP (Human): 100%; Urodilatin: 100%; ANP (Rat): 0.23%; BNP-32 (Human): 0.024%; Endothelin-1 (Human, Rat, Porcine, Mouse, Canine, Bovine): 0.01%; CNP (24-53): 0.009%; BNP-26 (Porcine): 0.006%; BNP-45 (Rat): 0%
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.