		Technical Data
A4156-17	Atrophin-1 Interacting Protein 4 (AIF4, ITCH, Itchy Homolog E3 Ubiquitin Protein Ligase (mouse), AIP4, NAPP1, dJ468O1.1, NFE2-associated Polypeptide 1, dJ468O1.1)

Description:
Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral andpallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. It can act as a transcriptional corepressor of p45/NFE2 and may participate in the regulation of immune responses by modifying Notch-mediated signaling. It is highly similar to the mouse Itch protein, which has been implicated in the regulation and differentiation of erythroid and lymphoid cells.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:32000
Western Blot: 0.03-0.1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

As reported

Immunogen:

Synthetic peptide of AIF4.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in Tris-saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

Specificity:

Recognizes human AIF4 at ~100kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 98.7kD according to NP_113671.3). Species Crossreactivity: Canine, mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Di Marcotullio L, Ferretti E, Greco A, De Smaele E, Po A, Sico MA, Alimandi M, Giannini G Maroder M, Screpanti I, Gulino A. Numb is a suppressor of Hedgehog signalling and targets Gli1 for Itch-dependent ubiquitination. Nat Cell Biol. 2006 Dec;8(12):1415-23. Epub 2006 Nov 19.