Technical Data
Atrophin-1 Interacting Protein 4 (AIF4, ITCH, E3 Ubiquitin-protein Ligase Itchy Homolog, AIP4, NAPP1, NFE2-associated Polypeptide 1, NAPP1)
ITCH is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It regulates the transcriptional activity of several transcription factors, and probably plays an important role in the regulation of immune response. ITCH down-regulates Epstein-Barr virus LMP2A activity in B cell signaling, and has been shown to interact via its WW domains with DRPLA, NFE2 and CBLC. Other potential interaction partners include NOTCH1, OCLN, JUN and JUNB.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.03-0.1ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human Brain (Frontal Cortex) lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
Synthetic peptide corresponding to C-EIKSHDLKPNGGN, from the internal region of the protein sequence according to NP_113671.3.
Purified by immunoaffinity chromatography.
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Recognizes human AIF4. Species sequence homology: canine, mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.