Technical Data
A4156-17D
Atrophin-1 Interacting Protein 4 (AIF4, ITCH, E3 Ubiquitin-protein Ligase Itchy Homolog, AIP4, NAPP1, NFE2-associated Polypeptide 1, NAPP1)
Description:
ITCH is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It regulates the transcriptional activity of several transcription factors, and probably plays an important role in the regulation of immune response. ITCH down-regulates Epstein-Barr virus LMP2A activity in B cell signaling, and has been shown to interact via its WW domains with DRPLA, NFE2 and CBLC. Other potential interaction partners include NOTCH1, OCLN, JUN and JUNB.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:32,000
Western Blot: 0.03-0.1ug/ml, observed in human brain, frontal cortex, lysates on ~100kD bands
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-EIKSHDLKPNGGN, from human ITCH, at the internal region of the protein (NP_113671.3).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:
Recognizes human ITCH. Species sequence homology: Canine, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Di Marcotullio L, Ferretti E, Greco A, De Smaele E, Po A, Sico MA, Alimandi M, Giannini G, Maroder M, Screpanti I, Gulino A. Numb is a suppressor of Hedgehog signalling and targets Gli1 for Itch-dependent ubiquitination. Nat Cell Biol. 2006 Dec;8(12):1415-23. Epub 2006 Nov 19.