Technical Data
A4159-04G
ATX1 (Ataxin 1)
Description:
Ataxin-1 is a member of the ATXN1 protein family and contains a single AXH domain. It is a neurodegenerative disorder protein thought to have a role in the metabolism of RNA as it has been shown to localize to the RNA and transcription dependent inclusions within the nucleus. A mutation of Ataxin-1 is the cause of spinocerebellar ataxia type-1 (SCA1), a progressive, neurodegenerative disease that is autosomal dominant and primarily affects the Purjinke cells found in brain stem neuronal populations and the cerebellum. Expression of Ataxin-1 is almost ubiquitous, except in the brain where it is isolated to populations of neurons.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: 1:500 dilution detects Ataxin-1 in rat cerebral cortex tissue.
Optimal dilutions to be determined by the researcher.

Positive Control:
Rat hippocampus tissue

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b,k11C231Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseMouse
Concentration:
~1mg/ml
Immunogen:
Linear peptide corresponding to mouse Ataxin-1. Cellular Localization: Cytoplasm (By similarity). Nucleus. Note: Co-localizes with USP7 in the nucleus (By similarity).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes mouse Ataxin-1. Species crossreactivity: rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Irwin, S., et al. (2005). J. Cell. Sci. 118:233- 242. 2. Bolger, T., et al. (2007). J. Biol. Chem. 282: 29186-29192.