Technical Data
A4165-04
AUF1 (A+U Rich RNA Binding Factor)
Description:
AUF1 Is a bcl-2 A + U-rich element-binding protein involved in bcl-2 mRNA destabilization during apoptosis.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.01-1ug/ml Detects AUF1 in RIPA lysates from HeLa nuclear extract and A431 cells. HeLa nuclear extract cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with A4165-04 (0.1ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.

Positive Control Included:
H1838: HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 5-10ul of extract and boil for 5 minutes to reduce the preparation. Load 20ug of reduces lysate per lane for minigels.

Recommended Secondary Antibodies
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ug4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Human AUF1 purified by Ni2+ affinity column
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.
Specificity:
Recognizes human AUF1 isoforms at Mr 37, 40, 42 and 45kD. Species sequence homology: Mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Zhang, et al., Mol. Cell. Biol. 13: 7652-7665, 1993.