		Technical Data
A4189-06H	AURKA, phosphorylated (Thr288) (Serine/Threonine-protein Kinase 6, Aurora Kinase A, Aurora/IPL1-related Kinase 1, ARK-1, Aurora-related Kinase 1, hARK1, Breast Tumor-amplified Kinase, Serine/Threonine-protein Kinase 15, Serine/Threonine-protein Kinase Aur

Description:
Aurora A plays a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. Aurora A plays a key role during tumor development and progression and is overexpressed in many human cancers including breast, ovarian and colorectal. Aurora A is viewed as a potential target for anticancer drug treatment.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry:1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

As reported

Immunogen:

Synthetic phosphopeptide corresponding to human AURKA around the phosphorylation site of threonine 288 (R-T-TP-L-M).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

Specificity:

Recognizes human AURKA when phosphorylated at Thr288. Species Cross-Reactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.