Technical Data
A4499-75
Avi Epitope Tag
Description:
Well-characterized antibodies for epitope tags have been widely used in the study of protein expression in various systems.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 0.05-0.2ug/ml
Western Blot: 0.1-1.0ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
40ug-20°CBlue IceRabbit
Concentration:
~0.5mg/ml (after reconstitution)
Immunogen:
Synthetic peptide corresponding to GLNDIFEAQKIEWHE from Avi tag conjugated to KLH.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 0.02% sodium azide. Reconstitute with 80ul sterile ddH2O.
Specificity:
Recognizes C-terminal, N-terminal and internal Avi tagged fusion proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.