Technical Data
A4500-06
Avidin
Description:
Avidin, synthesized in the hen oviduct, is a glycoprotein of MW 68,000 daltons which occupies about 0.05% of the total protein content of the hen egg white. Native avidin is a tetrameric protein composed of four identical subunits. Each subunit is glycosylated at 17-Asparagine.

Applications:
Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:2000-1:20,000
Western Blot: 1:1000-1:10,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
10mg-20°CBlue IceChickenRabbit
Concentration:
~10mg/ml (UV280)
Immunogen:
Avidin, Hen Egg White
Purity:
Puri ed from monospeci c antiserum by delipidation, fractionation and ion exchange chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Recognizes chicken Avidin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as puri ed and partially puri ed Avidin. Does not react with Streptavidin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.