		Technical Data
A4500-45	Avidin (HRP)

Description:
Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:7000 to 1:27,000. Assayed against 1ug of Avidin [Hen Egg White] in a standard capture ELISA using ABTS as a substrate for 30 minutes at RT.

Optimal dilutions to be determined by the researcher.

Storage and Stability:
For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Highly Purified

Size		Storage	Shipping	Source	Host
1mg		4°C (-20°C Glycerol)	Blue Ice	Chicken	Rabbit

Concentration:

~10mg/ml (UV280)

Immunogen:

Avidin (Hen Egg White).

Purity:

Puri ed by delipidation, fractionation and ion exchange chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 10 mg/ml BSA (IgG and Protease free), 0.01% Gentamicin Sulfate, 40% glycerol. Labeled with HRP.

Specificity:

Recognizes chicken Avidin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as puri ed and partially puri ed Avidin (Hen Egg White). Crossreactivity against Avidin from other sources is unknown.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.