Technical Data
A4500-46
Avidin (HRP)
1mg
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Technical Data |
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A4500-46 |
Avidin (HRP) |
1mg |
| BioSeparation? | Storage: -20°CShipping: Blue Ice |
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Avidin, synthesized in the hen oviduct, is a glycoprotein of MW 68,000 daltons which occupies about 0.05% of the total protein content of the hen egg white. Native avidin is a tetrameric protein composed of four identical subunits. Each subunit is glycosylated at 17-Asparagine. Applications: Suitable for ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:20,000-1:200,000 Western Blot: 1:10,000- 1:40,000. Immunohistochemistry: 1:1000-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light. |
Purity: Chromatographically purified. IEP Assay shows a single precipitin arc against anti-Avidin and anti-Peroxidase. Concentration: ~2mg/ml Form: Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, (IgG and protease free), 0.01% gentamicin sulfate. Labeled with Horseradish Peroxidase (HRP). Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
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