Technical Data
A4500-81F
Avidin D (Biotin)
Description:
Biotinylated Anti-Avidin D is an unusual molecule. It is capable of binding to Avidin D by two mechanisms, either through the antigen binding sites in the Fab portion of the antibody or through multiple biotin residues covalently attached to the protein. Although little is known about the mechanism of binding, this reagent appears to provide superior amplification of fluorescent signals compared to biotinylated "extender" molecules or anti-avidin alone. The steps involved in using this product are straightforward. After a biotinylated antibody has been introduced, cells or sections are labeled with Fluorescein Avidin DCS, followed by Biotinlyated Anti-Avidin D, and then a second labeling with Fluorescein Avidin DCS. This procedure results in the introduction of several more fluorochromes at the antigenic site in the tissue. Cell sorter analysis has shown that a several-fold increase in fluorescence can be achieved in some antibody labeling studies using Biotinylated Anti-Avidin D. Other applications of this unique reagent are currently under investigation.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1-10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceGoat
Concentration:
Not determined
Immunogen:
Purity:
Affinity Purified
Form
Supplied as a powder in 10mM HEPES, pH 7.5, 0.15M sodium chloride, 0.08% sodium azide. Reconstitute with 1ml dH2O. Labeled with Biotin.
Specificity:
Recognizes Avidin D.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Simmons PJ, Przepiorka D, Thomas ED, and Torok-Storb B: Host Origin of Marrow Stromal Cells Following Allogeneic Bone Marrow Transplantation. Nature. Vol. 328,429-432, 1987. Przepiorka D and Myerson D: A Single-step Silver Enhancement Method Permitting Rapid Diagnosis of Cytomegalovirus Infection in Formalin-fixed, Paraffin-embedded Tissue Sections by In Situ Hybridization and Immunoperoxidase Detection. J. Histochem. Cytochem. Vol. 34,1731-I734, 1986. Pinkel D, Straume T and Gray JW: Cytogenetic Analysis Using Quantitative, High-sensitivity Fluorescence Hybridization. Proc. Natl. Acad. Sci. Vol. 83,2934-2938, 1986. Phillips HS, Nikolics K, Branton D and Seeburg PH: Immunocytochemical Localization in Rat Brain of a Prolactin Release-Inhibiting Sequence of Gonadotropin-Releasing Hormone Prohormone. Nature. Vol. 316,542-545, 1985.