Technical Data
A4745
Axin, aa898-912
Description:
Axin, an important regulator of beta-catenin, is frequently mutated in human hepatocellular carcinomas (HCCs), and transduction of the wild-type Axin gene (AXIN1) induces apoptosis in HCC cells as well as in colon cancer cells. A novel gene, AXUD1 (AXIN 1 up-regulated), has enhanced expression in response to exogenously expressed AXIN1. It is expressed in all human tissues examined, most abundantly in lung, placenta, skeletal muscle, pancreas and leukocytes.

Applications
Western Blotting: 1-2ug/ml detectsAxin in RIPA lysates from Cos-1 cells transfected with full length, mouse axin. Lysates of Cos-1 cells untransfected (lane 1) and expressing transfected Axin (lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Axin (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ug4C (-20C Glycerol)Blue IceMouseRabbit
Concentration:
~1mg/ml
Immunogen:
KLH-conjugated, synthetic peptide corresponding to amino acids 898-912 (SQRKAGGGSAPPCDS) of mouse axin. The immunizing sequence is identical in rat and has 13/15 identical amino acids in human.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
Specificity:
Recognizes recombinant mouse Myc-tagged Axin expressed in transfected cell lysate, Mr 130kD. Predicted to crossreact with rat and human based on sequence homology.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Kikuchi, A., Cell Signal 11: 777, 1999