Technical Data
A4747
Axin 1 (Axis Inhibitor 1, MGC52315)
Description:
Axin, an important regulator of beta-catenin, is frequently mutated in human hepatocellular carcinomas (HCCs), and transduction of the wild-type Axin gene (AXIN1) induces apoptosis in HCC cells as well as in colon cancer cells. A novel gene, AXUD1 (AXIN 1 up-regulated), has enhanced expression in response to exogenously expressed AXIN1. It is expressed in all human tissues examined, most abundantly in lung, placenta, skeletal muscle, pancreas and leukocytes.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:32,000.
Western Blot: No signal obtained yet but low background observed in Human Brain, Human Pancreas, MOLT-4 and HeLa lysates at up to 2.0ug/ml.

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Peptide with sequence FEEKIIGKVEKVD, from C Terminus of the protein sequence according to NP_003493; NP_851393.
Purity:
Purified from goat serum by ammonium sulfate precipitation followed by immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes AXIN 1. Species Crossreactivity: Expected to crossreact with Human, Mouse and Rat due to sequence homology.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Zeng L et al. The mouse Fused locus encodes Axin, an inhibitor of the Wnt signaling pathway that regulates embryonic axis formation. Cell 90:181-92 (1997).