Technical Data
A4747A
Axin (Axis Ihibition Protein)
Description:
The Axin (axis inhibition) protein is an ~120-130kD negative regulator of Wnt pathway signaling. The Wnt pathway is essential for embryonic axis development in amphibians, Drosophila, and mammals; Axin has been shown to inhibit dorsal axial development. In vertebrates, Wnt signaling occurs when the frizzled receptor transduces a signal to the Dishevelled protein, triggering glycogen synthase kinase-3 (GSK-3) inactivation and the accumulation of b-catenin. Axin binds directly to GSK-3b, b-catenin, and the adenomatous polyposis coli (APC) tumor suppressor protein at separate binding sites; these four proteins form a tetrameric complex that regulates b-catenin stability and signaling.

Applications:
Suitable for use in Western Blot. Other applications have not been tested.

Recommended Dilution:
Western Blot: 0.1-2.0ug/ml. Detects a band at ~120-130kD using Jurkat leukemia T cell lysates.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.25mg/ml
Immunogen:
Synthetic peptide derived from the internal region of the human Axin protein. Based on amino acid sequence, this antibody is not expected to cross-react with Axin-2.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes human Axin. On Western blots, it recognizes a doublet band at ~120-130kD. Species sequence homology: mouse, rat, and chicken-86%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.