Technical Data
A4775-50C
Axl (AXL Oncogene, AXL Receptor Tyrosine Kinase, AXL Transforming Gene, AXL Transforming Sequence/gene, Adhesion-related Kinase, Ark, Oncogene AXL, Tyrosine Protein Kinase Receptor, UFO, TYR07)
Description:
AXL is an 887aa proto-oncogene belonging to the protein kinase super family with a protein kinase domain, two Ig-like C2-type (immunoglobulin-like) domains and two fibronectin type–III domains. Unlike other receptor tyrosine kinases, AXL represents a unique novel structure in the extracellular region that juxtaposes IgL and FNIII repeats. AXL may function as a signal transducer between specific cell types of mesodermal origin and may transduce signals from the extracellular matrix into the cytoplasm by binding to growth factors like vitamin K-dependent protein growth-arrest-specific gene 6 (GAS6) and thus form heterodimer and heterotetramer. It plays an important role in the stimulation of cell proliferation and can also mediate cell aggregation by homophilic binding. In case of filovirus infection, AXL seems to function as a cell entry factor. Studies reveal the transforming potential of AXL in patients with chronic myeloproliferative disorder or chronic myelocytic leukemia..

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
HUVEC cell lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa450-500 of human AXL/UFO.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity:
Recognizes human AXL. Species Crossreactivity: mouse and rat. Species sequence homology: porcine, xenopus.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Janssen J.W.G. et al. Oncogene. 6:2113-2120 (1991). 2. Liu E. et al. Proc. Nat. Acad. Sci. 85:1952-1956 (1988). 3. O'Bryan J.P. et al. Molec. Cell. Biol. 11:5016-5031 (1991). 4. Varnum B.C. et al. Nature. 373:623-626 (1995).