Technical Data
A4776-10
Axl, phosphorylated (Tyr779) (AXL Oncogene, AXL Receptor Tyrosine Kinase, AXL Transforming Gene, AXL Transforming Sequence/gene, Adhesion-related Kinase, Ark, Oncogene AXL, Tyrosine Protein Kinase Receptor, UFO, TYR07)
Description:
Axl (also known as ARK, UFO and TYR07) is a receptor tyrosine kinase that contains extracellular regions composed of two IgG-related domains linked to two fibronectin type III repeats. Gas6, a ligand for Axl, induces Axl phosphorylation on multiple tyrosine residues. Phosphorylation of Axl at Tyr779 is associated with the activation of PI 3-kinase.

Applications:
Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1ug/ml detects Axl in Wl38 human lung cell line conditioned media-treated A172 human glioblastoma cell line.
Immunocytochemistry: 5-15ug/ml detects Axl in immersion fixed A172 human glioblastoma cell line with and without pervanadate co-staining with total Axl.
Immunohistochemistry: 5-15ug/ml detects Axl in immersion fixed paraffin embedded sections of human stomach.
Optimal dilution to be determined by researcher.

Recommended Secondary Antibodies:
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb
I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.2mg/ml (after reconstitution)
Immunogen:
Synthetic phosphopeptide corresponding to human Axl Tyr779 site.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity:
Recognizes endogenous human Axl, phosphorylated at Tyr779. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.