Technical Data
A4776-10E
Axl (AXL Oncogene, AXL Receptor Tyrosine Kinase, AXL Transforming Gene, AXL Transforming Sequence/gene, Adhesion-related Kinase, Ark, Oncogene AXL, Tyrosine Protein Kinase Receptor, UFO, TYR07)
Description:
AXL receptor, an 894aa novel Type-I transmembrane protein, is a member of Cell adhesion molecule-related receptor tyrosine kinase family. A chronic myelogeneous leukemia-associated Oncogene, AXL is composed of unique 2 immunoglobulin-like and 2 Fibronectin III repeats in the extracellular region and a C-terminal tyrosine kinase domain. A multi-functional protein mediating Gas6 induced signal transduction; AXL receptor mediates anti-apoptosis, cell proliferation and survival through STAT3 activation, Akt phosphorylation, NF-B activation, Bcl-2 expression and caspase-3 inhibition. Gas6-Axl interactions are involved in regulation of multiple tissue processes including vascular remodeling, mesanglial cell proliferation in kidney, up regulating osteoclast function, protecting neurons from amyloid induced apoptosis in brain, mediating outer retinal pigment epithelial function in eye and cell adhesion during hematopoiesis. AXL receptor is over expressed in many cancers and diseases indicating a potential target for cancer, renal and anti-thrombotic therapies.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined.
Immunogen:
Synthetic peptide corresponding to aa785-801 (CWELNPQDRPSFTELRE) of human AXL receptor.
Purity:
Serum.
Form
Supplied as a liquid, 0.025% sodium azide, 50% glycerol.
Specificity:
Recognizes human AXL receptor/UFO. Species sequence homology: bovine, canine, mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Inhibition of vascular endothelial growth factor receptor 2-mediated endothelial cell activation by Axl tyrosine kinase receptor. Blood 105 (5), 1970-1976 (2005). 2. van Ginkel,P.R., Gee,R.L., Shearer,R.L., Subramanian,L., Walker,T.M., Albert,D.M., Meisner,L.F., Varnum,B.C. and Polans,A.S. Expression of the receptor tyrosine kinase Axl promotes ocular melanoma cell survival. Cancer Res. 64 (1), 128-134 (2004). 3. Sainaghi,P.P., Castello,L., Bergamasco,L., Galletti,M., Bellosta,P. and Avanzi,G.C. Gas6 induces proliferation in prostate carcinoma cell lines expressing the Axl receptor. J. Cell. Physiol. 204 (1), 36-44 (2005). 4. Jannsen JW et.al Oncogene 6(11):2113-20 (1991 Nov).