Technical Data
A8510
ASIALOGLYCOPROTEIN RECEPTOR (ASGPR)
Description:
The asialoglycoprotein receptor (ASGPR) is a transmembrane glycoprotein (42kD) which mediates binding, internalisation and degradation of extracellular glycoproteins that have exposed terminal galactose residues. The receptor is expressed on the surface of the hepatocytes in a polar manner, i.e. it is present on the sinusoidal and lateral plasma membranes but not on the bile canalicular membrane. The mammalian hepatic ASGPR mediates the endocytosis and degradation of serum proteins from which terminal sialic residues have been removed.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry, Immunofluorescence, ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:50
Western Blot: 1:50 Detects a band at ~42kD. A non-reduced sample treatment and SDS-PAGE followed by blotting on nitrocellulose or PDFM is recommended. Block with 10% non-fat dry milk.
Immunohistochemistry (frozen): 1:50 Acetone-fixed
ELISA: 20ug/ml using 100ul/well
Optimal dilutions to be determined by the researcher.

Recommended Positive Control:
Rat hepatocytes

Recommended Negative Control:
Rat Kidney, pancreas, small intestine, colon

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG19G132Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatMouse
Concentration:
~0.1mg/ml
Immunogen:
Crude rat liver membrane extracts
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Specificity:
Recognizes a subunit-specific epitope on RHL-1 of rat ASGPR. Species crossreactivity: human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Shimada, M et al; A monoclonal antibody to rat asialoglycoprotein receptor that recognizes an epitope specific to its major subunit. Hepatol Res 2003, 26: 55 2. Mizuno, M et al; Monoclonal antibodies identifying antigens on distinct domains of rat hepatocytes. Liver 1987, 7: 251 3. Hyodo, I et al; Distribution of asioalogycoprotein receptor in human hepatocellular carcinoma. Liver 1993, 13: 80 4. Mizuno, M et al: Development of a monoclonal antibody identifying an antigen which is segregated to the sinusoidal and lateral plasma membranes of rat hepatocytes. Gastroenterol Japan 1986, 21: 238