Technical Data
The asialoglycoprotein receptor (ASGPR) is a transmembrane glycoprotein (42kD) which mediates binding, internalisation and degradation of extracellular glycoproteins that have exposed terminal galactose residues. The receptor is expressed on the surface of the hepatocytes in a polar manner, i.e. it is present on the sinusoidal and lateral plasma membranes but not on the bile canalicular membrane. The mammalian hepatic ASGPR mediates the endocytosis and degradation of serum proteins from which terminal sialic residues have been removed.

Suitable for use in Flow Cytometry, Immunohistochemistry, Immunofluorescence, ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:50
Western Blot: 1:50 Detects a band at ~42kD. A non-reduced sample treatment and SDS-PAGE followed by blotting on nitrocellulose or PDFM is recommended. Block with 10% non-fat dry milk.
Immunohistochemistry (frozen): 1:50 Acetone-fixed
ELISA: 20ug/ml using 100ul/well
Optimal dilutions to be determined by the researcher.

Recommended Positive Control:
Rat hepatocytes

Recommended Negative Control:
Rat Kidney, pancreas, small intestine, colon

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG19G132Affinity Purified
100ug-20CBlue IceRatMouse
Crude rat liver membrane extracts
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Recognizes a subunit-specific epitope on RHL-1 of rat ASGPR. Species crossreactivity: human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Shimada, M et al; Hepatol Res 2003, 26: 55 2. Mizuno, M et al; Liver 1987, 7: 251 3. Hyodo, I et al; Liver 1993, 13: 80 4. Mizuno, M et al: Gastroenterol Japan 1986, 21: 238. 5. Hirai, M et al. Acta med okayama 2002 56: 135. 6. Keyel, P et al. Mol boil Cell 2006 17:4300. 7. Zhou, X et al. Hum Reprod 2009 1: 1.