Technical Data
A8510-01
ASIALOGLYCOPROTEIN RECEPTOR (ASGPR) (FITC)
Description:
The asialoglycoprotein receptor (ASGPR) is a transmembrane glycoprotein (42kD) which mediates binding, internalisation and degradation of extracellular glycoproteins that have exposed terminal galactose residues. The receptor is expressed on the surface of the hepatocytes in a polar manner, i.e. it is present on the sinusoidal and lateral plasma membranes but not on the bile canalicular membrane. The mammalian hepatic ASGPR mediates the endocytosis and degradation of serum proteins from which terminal sialic residues have been removed.

The rat asialoglycoprotein receptor (ASGPR) consists of three polypeptide subunits: rat hepatic lectin (RHL)-1, RHL-2 and RHL-3.

Applications:
Suitable for use in Flow Cytometry, Immunofluorescence, Immunohistochemistry, ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:50
Western Blot (Non-reduced): 1:50
Immunohistochemistry (frozen, aceton fixed): 1:50
Optimal dilutions to be determined by the researcher.

Recommended Positive Control:
Rat hepatocytes

Recommended Negative Controls:
Rat kidney, pancreas, small intestine, colon

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG19G133Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatMouse
Concentration:
~0.1mg/ml
Immunogen:
Crude rat liver membrane extracts.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Specificity:
Recognizes a subunit-specific epitope on RHL-1 of rat ASGPR. at 42kD. Species Crossreactivity: human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Shimada, M et al; A monoclonal antibody to rat asialoglycoprotein receptor that recognizes an epitope specific to its major subunit. Hepatol Res 2003, 26: 55 2. Mizuno, M et al; Monoclonal antibodies identifying antigens on distinct domains of rat hepatocytes. Liver 1987, 7: 251 3. Hyodo, I et al; Distribution of asioalogycoprotein receptor in human hepatocellular carcinoma. Liver 1993, 13: 80 4. Mizuno, M et al: Development of a monoclonal antibody identifying an antigen which is segregated to the sinusoidal and lateral plasma membranes of rat hepatocytes. Gastroenterol Japan 1986, 21: 238