Technical Data
B0000-35A
B7H4 (B7-H4, B7S1, B7x, BC032925, Immune Costimulatory Protein B7H4, MGC41287, PRO1291, T Cell Costimulatory Molecule B7x, V Set Domain-containing T Cell Activation Inhibitor 1, VCTN1)
Description:
As a member of B7 family, B7-H4 (VTCN1) plays a critical role in the regulation of antigen-specific immune responses. B7-H4 is a co-stimulatory protein expressed in activated T cells and is involved in the negative regulation of cell-mediated immunity in peripheral tissues. B7-H4 ligation to its receptor BTLA T-lymphocytes results in inhibition of T-cell activation, cytokine secretion, and the development of cytotoxicity. B7-H4 protein is typically expressed on the surface of activated T-cells, B-cells, monocytes, and dendritic cells, but not in most normal tissues. In tissues, B7-H4 is over expressed in breast, endometrial, ovarian, renal cell (RCC), and non-small cell lung carcinomas (NSCLC). B7-H4 has been identified as an important cancer marker for breast and ovarian cancers.

Applications:
Suitable for use in Peptide ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Peptide ELISA: 1:64,000 (Detection limit dilution)
Western Blot: 0.5-1.5ug/ml. Detects an ~28kD band in human pancreas lysates.
Optimal dilutions to be determined by the researcher.

Control Peptide:
B0000-35A-P

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-SKGKGNANLEYK, from the internal region of human B7H4 (NP_078902.2). Species Sequence Homology: mouse, rat, canine.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human B7H4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Krambeck, A.E., et al., Proc. Natl. Acad. Sci. USA 103(27): 10,391-10,396 (2006).