Technical Data
B7H4 (B7-H4, B7S1, B7x, BC032925, Immune Costimulatory Protein B7H4, MGC41287, PRO1291, T Cell Costimulatory Molecule B7x, V Set Domain-containing T Cell Activation Inhibitor 1, VCTN1) (PE)
B7H4, also known as B7x, is a costimulatory protein which is reported to function as a negative regulator of T-cell mediated immunity. Although B7H4 binds an unknown receptor, it is thought to deliver an inhibitory signal to T-cells preventing their proliferation, cell cycle progression and interleukin-2 production. B7H4 deficient mice are only minimally affected; suggesting B7H4 is important in the fine tuning of the T-cell mediated immune response. B7H4 is expressed on activated T-cells, B-cells, monocytes and dendritic cells. Aberrant expression has been associated with cancers of the lung, breast and ovary in humans. B7-H4 could be a useful prognostic marker in Renal Cell Carcinoma (RCC).

Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: Neat. 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Recommended Negative Control:
I1904-79D: IgG1 Murine Negative Control (PE)

Storage and Stability:
Store product at 4°C before and after reconstitution. DO NOT FREEZE!  Lyophilized and reconstituted products are stable for 12 months at 4°C after receipt. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid.
Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.
MabIgG110B249Affinity Purified
100Tests4°C Do Not FreezeBlue IceHumanMouse
Not Determined
Human B7H4
Purified by Protein G affinity chromatography.
Supplied as a lyophilized powder from PBS, 5% sucrose, 1% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O.
Recognizes human B7H4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. van de Ven, R., et al., Blood 118: 2502-2510 (2011).