Technical Data
B0000-35B5
B7H4 (B7-H4, B7S1, B7x, BC032925, Immune Costimulatory Protein B7H4, MGC41287, PRO1291, T Cell Costimulatory Molecule B7x, V Set Domain-containing T Cell Activation Inhibitor 1, VCTN1) (PE)
Description:
B7H4, also known as B7x, is a costimulatory protein which is reported to function as a negative regulator of T-cell mediated immunity. Although B7H4 binds an unknown receptor, it is thought to deliver an inhibitory signal to T-cells preventing their proliferation, cell cycle progression and interleukin-2 production. B7H4 deficient mice are only minimally affected; suggesting B7H4 is important in the fine tuning of the T-cell mediated immune response. B7H4 is expressed on activated T-cells, B-cells, monocytes and dendritic cells. Aberrant expression has been associated with cancers of the lung, breast and ovary in humans. B7-H4 could be a useful prognostic marker in Renal Cell Carcinoma (RCC).

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: Neat. 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Recommended Negative Control:
I1904-79D: IgG1 Murine Negative Control (PE)

Storage and Stability:
Store product at 4°C before and after reconstitution. DO NOT FREEZE!  Lyophilized and reconstituted products are stable for 12 months at 4°C after receipt. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid.
Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG110B249Affinity Purified
SizeStorageShippingSourceHost
100Tests4°C Do Not FreezeBlue IceHumanMouse
Concentration:
Not Determined
Immunogen:
Human B7H4
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 5% sucrose, 1% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O.
Specificity:
Recognizes human B7H4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. van de Ven, R., et al., Blood 118: 2502-2510 (2011).