Technical Data
B0001-53
B Cells (FITC)
Description:
Recommended Negative Controls: Mouse IgM Negative Control: FITC

Applications:
Flow Cytometry: Neat
Optimal working dilutions to be determined by researcher.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells or 100ul whole blood.
TypeIsotypeCloneGrade
MabIgM3H565Highly Purified
SizeStorageShippingSourceHost
100Tests-20CBlue IceHumanMouse
Concentration:
~100 Tests/1ml
Immunogen:
HRIK cells - Human B-Lymphoblastoid line.
Purity:
Purified by gel filtration.
Form
Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid in 0.1M Tris-HCL, 0.5ml NaCl, 10mM glycine pH 8.0 with 0.1% sodium azide, 1% BSA.
Specificity:
3H565 recognizes a glycoprotein antigen of 105kD expressed by B lymphocytes. The 3H565 antigen is expressed by peripheral B lymphocytes and has been used widely in the study of B cell malignancy. 3H565 has been used extensively to differentiate various types of B cell malignancy. B-CLL is generally considered to be negative for 3H565 expression, but strong staining is seen in many other types of B cell lymphoma, including prolymphocytic leukaemia and hairy cell leukaemia. The nature of the 3H565 antigen has remained poorly defined following its first description in 1981. The expression pattern closely corresponds to that seen with CD22, but studies have shown that 3H565 does not recognize the CD22 molecule itself. A recent publication by Serke et al has shown that 3H565 recognizes a conformational epitope on the CD20 molecule, most likely a multimeric complex of CD20.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Brooks D.A., et al. (1981). Human lymphocyte markers defined by antibodies derived from somatic cell hybrids. A monoclonal antibody reacting specifically with a subpopulation of human B lymphocytes. J. Immunol. 126: 1373.2. Cato