		Technical Data
B0001-65	B Cell Subset

Description:
While identification of the antigen(s) that are found predominantly on ILPP B cells and which are recognized by this antibody is speculative, the antibody appears to react with an immature population of B cells in the follicles of ILPPs and with a subpopulation of sIglow B cells in lymph nodes.

Applications:
1. Flow cytometry 1
2. Immunohistochemistry (acetone-fixed, frozen tissue sections only) 1

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgM,k	5K110	Purified

Size		Storage	Shipping	Source	Host
500ug		-20°C	Blue Ice	Porcine	Mouse

Concentration:

~0.5mg/ml

Immunogen:

Purity:

Purified

Form

Supplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.

Specificity:

Recognizes porcine B cell subset. Stains the following percentages of surface immunoglobulin (sIg) positive cells in porcine lymphoid tissues: 11-15% in peripheral blood; 22-23% in mesenteric lymph nodes; 79-87% in ileal Peyer’s patches (ILPP); 6-17 in spleen; and 0% in thymus.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Denham, S., R.J. Zwart, J.T.D. Whittall, M. Pampusch, A.H. Corteyn, A.T.J. Bianchi, M.P. Murtaugh, R.M.E. Parkhouse, H. Tlaskalova, J. Sinkora, M. Siinkora, and Z. Rehakova. 1998. Monoclonal antibodies putatively identifying porcine B cells. Veter. Immunol. Immunopathol. 60:317-328.