Technical Data
Bacillus anthracis LF (Lethal Factor) (Anthrax)
Anthrax PA (Protective Antigen) and Anthrax LF (Lethal Factor), along with Anthrax EF (Edema Factor), are toxins produced by Bacillus anthracis. All three of the proteins must be present for toxicity to occur. It begins with the binding of PA to its cell-surface receptor, ATR (Anthrax Toxin Receptor), at which point PA is cleaved and the resulting 63kD carboxyl-terminal fragment binds to LF and EF. LF is a protease that is known to cleave members of the mitogen-activated protein kinase kinase (MAPKK) family (2).

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Recommended Positve Control:
Recombinant protein

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
200ul4C (-20C Glycerol)Blue IceRabbit
Not determined
Synthetic peptides corresponding to aa779-792 of the Anthrax LF protein (~90kD)
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Recognizes Anthrax LF (Lethal Factor) Protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Collier, R.J. & Young, J.A., Annu. Rev. Cell Dev. Biol. 19: 45-70 (2003). 2. Cunningham, K., et al., Proc. Natl. Acad. Sci. USA 99(10): 7049-7053 (2002). 3. Lacy, D.B, et al., J. Biol. Chem. 277(4): 3006-3010 (2002).