Technical Data
Bacillus anthracis LF (Lethal Factor) (Anthrax)
Anthrax PA (Protective Antigen) and Anthrax LF (Lethal Factor), along with Anthrax EF (Edema Factor), are toxins produced by Bacillus anthracis. All three of the proteins must be present for toxicity to occur. It begins with the binding of PA to its cell-surface receptor, ATR (Anthrax Toxin Receptor), at which point PA is cleaved and the resulting 63kD carboxyl-terminal fragment binds to LF and EF. LF is a protease that is known to cleave members of the mitogen-activated protein kinase kinase (MAPKK) family2.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1ug/ml
Optimal dilution to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG14H27Affinity Purified
100ug4C (-20C Glycerol)Blue IceMouse
Synthetic peptide corresponding to amino acids 779-792 of the Anthrax LF protein (~90 kD).
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Recognizes Anthrax LF (Lethal Factor) Protein
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Collier, R.J. & Young, J.A., Annu. Rev. Cell Dev. Biol. 19: 45-70 (2003). 2. Cunningham, K., et al., Proc. Natl. Acad. Sci. USA 99(10): 7049-7053 (2002). 3. Lacy, D.B, et al., J. Biol. Chem. 277(4): 3006-3010 (2002).