Technical Data
Bacillus anthracis PA (Protective Antigen) (Anthrax)
Anthrax PA (Protective Antigen) and Anthrax LF (Lethal Factor), along with Anthrax EF (Edema Factor), are toxins produced by Bacillus anthracis. All three of the proteins must be present for toxic entry into cells. PA is secreted from Bacillus anthracis as an 83kD (PA83) protein which is cleaved by furin protease, releasing a 20kD fragment. The 63kD portion (PA63) of the cleavage product forms a heptamer, which binds the toxins LF and EF. This complex enters via receptor-mediated endocytosis where LF and EF exert their toxic effects.
PA is cleaved from the 83kD form to the 63kD form at aa164-167. Thus the antibody (made against aa186-192) should recognize both the 83kD and 63kD PA forms, but not the 20kD cleavage fragment.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000
Optimal dilutions to be determined by researcher.

Recommended Positive Control:
PA recombinant protein

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
200ul-20CBlue IceRabbit
Not Determined
Synthetic peptides corresponding to aa186-192 of the Anthrax PA protein (~83kD).
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Recognizes Anthrax PA (Protective Antigen) protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Collier, R.J. & Young, J.A., Annu. Rev. Cell Dev. Biol. 19: 45-70 (2003). 2. Cunningham, K., et al., PNAS USA 99(10): 7049-7053 (2002, May 14. Epub: 2002, May 07). 3. Lacy, D.B., et al., J. Biol. Chem. 277(4): 3006-3010 (2002).