Technical Data
Bacillus anthracis Edema Factor (Anthrax) (Biotin)
After inhalation by mammals, Bacillus anthracis spores germinate in alveolar macrophages then migrate to lymph nodes where they multiply. The vegetative bacteria excrete the tripartite exotoxin which consists of three polypeptides: protective antigen (PA, 83kD), lethal factor (LF, 90kD) and oedma factor (OF, 89kD). The two components (OF/EF and LF) of the toxin enzymatically modify substrates within the cytosol of the mammalian cells: The OF/EF is an adenylate cyclase that impairs the host defenses through a variety of mechanisms inhibiting phagocytosis. The LF is a zinc dependent protease that cleaves several mitogen activated protein kinase kinases (MAPKK) and causes lysis of macrophages. To intoxicate mammalian cells, the third component of the toxin PA, binds to a ubiquitously expressed cellular receptor, Tumor Endothelium Marker 8 (TEM8). Upon binding to TEM8, PA is cleaved into 20 and 63kD fragments (PA20 and PA63) by furin or furin-like proteases. PA20 dissociates into medium and allows the PA63 fragment to heptamerize and bind LF and OF of the toxin. The resulting complex of PA63 fragment with EF and/or OF binds to PA-receptor TEM8/ATR and internalized into endosomes followed by translocation of LF and OF into cytosol of the cells.

B. anthracis Edema Factor (LF) is one of the three proteins that comprise anthrax toxin, the others being lethal factor (LF), and protective antigen (PA). Edema factor is a calmodulin-dependent adenylate cyclase that is responsible for the swelling in cutaneous anthrax infections. The cellular cAMP levels increases in host cells since the edema factor forms a complex with the host calmodulin which stimulates the activity of EF. The EF also inhibits the immune response by removing calmodulin from involvement in calcium triggered signaling.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 0.1-2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
50ug-20CBlue IceGoat
As reported
Recombinant corresponding to purified Bacillus anthracis Edema Factor (EF).
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH7.4, 0.1% BSA, 0.1% Proclin-300. Labeled with Biotin.
Recognizes Bacillus anthracis Edema Factor. Does not cross-react with Protective Antigen (PA) of Bacillus anthracis, Y. Pestis, F. Tularensis and Toxoplasma gondi.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Arun P. Chopra et al (2003) JBC Vol. 278, Issue 11, 9402-9406. 2. Sung O et al (2003) JBC Vol. 278, 7413-7421. 3. Bradley KA et al (2001) Nature 414, 225-229. 4. liu S and Leppla SH (2002) JBC (in press). 5. Leppla, SH (1982) PNAS 79, 3182. 6. O'Brien J et al (1985) Infect Immun 47, 306.